Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anacardic acid inhibits the proliferation and inflammation of HaCaT cells induced by TNF-α via the regulation of NF-κB pathway

Tao Liu , Yuanmin He, Yongmei Liao

Department of Dermatology, Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province 646000, China;

For correspondence:- Tao Liu Email: TaoLiufjk@163.com Tel:+8618982496851

Accepted: 2 February 2024 Published: 29 February 2024

Citation: Liu T, He Y, Liao Y. Anacardic acid inhibits the proliferation and inflammation of HaCaT cells induced by TNF-α via the regulation of NF-κB pathway. Trop J Pharm Res 2024; 23(2):251-256 doi: 10.4314/tjpr.v23i2.3

© 2024 The authors.
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Abstract

Purpose: To determine the effect of anacardic acid on HaCaT cells in vitro and to elucidate its molecular action.

Methods: HaCaT cells were incubated in varying concentrations of anacardic acid (10 to 50 µM). To model psoriasis, the cells were treated with tumor necrosis factor-α (TNF-α); cell viability was gauged by CCK-8 assay. Apoptosis was determined, and Bcl-2, Bax, p65, p-p65, p-IκBα and IκBα by Western blot. Levels of pro-inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA).

Results: Anacardic acid resulted in reduced HaCaT cell viability and increased cell apoptosis in a concentration-dependent manner (p < 0.05). It also curtailed TNF-α-mediated inflammatory responses and downregulated the NF-κB signaling axis.

Conclusion: The results indicate that anacardic acid impedes HaCaT cell growth and inflammatory cytokine production by interfering with NF-κB signal transduction, and may influence the development of AA-based therapies for psoriasis.

Keywords: Anacardic acid, Psoriasis, Keratinocytes, NF-κB, Skin diseases